2O1B
Structure of aminotransferase from Staphylococcus aureus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9795, 0.9796, 0.9642 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.311, 56.307, 73.401 |
Unit cell angles | 90.00, 130.31, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.18071 |
Rwork | 0.178 |
R-free | 0.22633 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.250 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.079 | 0.197 |
Number of reflections | 21132 | |
<I/σ(I)> | 11.6 | 2.8 |
Completeness [%] | 89.2 | 77.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |