2O0Y
Crystal structure of putative transcriptional regulator RHA1_ro06953 (IclR-family) from Rhodococcus sp.
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-11 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.247, 69.027, 79.214 |
| Unit cell angles | 74.88, 71.47, 68.87 |
Refinement procedure
| Resolution | 36.250 - 2.000 |
| R-factor | 0.18482 |
| Rwork | 0.182 |
| R-free | 0.23384 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.617 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.244 |
| Number of reflections | 73258 | |
| <I/σ(I)> | 36 | 6.6 |
| Completeness [%] | 97.3 | 91.1 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG3350 25%, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
