2O0L
Human spermidine synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-13 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.323, 60.593, 86.667 |
| Unit cell angles | 90.00, 108.96, 90.00 |
Refinement procedure
| Resolution | 81.920 - 1.990 |
| R-factor | 0.20923 |
| Rwork | 0.206 |
| R-free | 0.26755 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 81.920 |
| High resolution limit [Å] | 1.990 |
| Rmerge | 0.121 |
| Number of reflections | 36969 |
| <I/σ(I)> | 7.5 |
| Completeness [%] | 99.4 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 20% PEG 3350, 0.2M Mg(OAc)2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
