2O0I
crystal structure of the R185A mutant of the N-terminal domain of the Group B Streptococcus Alpha C protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 55.700, 55.700, 277.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.840 - 3.100 |
| R-factor | 0.247 |
| Rwork | 0.245 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ywm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.610 | 3.100 |
| Rmerge | 0.143 | 0.058 | 0.347 |
| Number of reflections | 5264 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 100.0 | 100 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | 0.1M sodium acetate, 10% PEG4000, DTT, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
