2O0I
crystal structure of the R185A mutant of the N-terminal domain of the Group B Streptococcus Alpha C protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-13 |
Temperature [K] | 100 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 55.700, 55.700, 277.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.840 - 3.100 |
R-factor | 0.247 |
Rwork | 0.245 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ywm |
RMSD bond length | 0.011 |
RMSD bond angle | 1.639 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 3.210 |
High resolution limit [Å] | 3.100 | 6.610 | 3.100 |
Rmerge | 0.143 | 0.058 | 0.347 |
Number of reflections | 5264 | ||
<I/σ(I)> | 5.7 | ||
Completeness [%] | 100.0 | 100 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | 0.1M sodium acetate, 10% PEG4000, DTT, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |