2O0A

The structure of the C-terminal domain of Vik1 has a motor domain fold but lacks a nucleotide-binding site.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-20
Detector	CUSTOM-MADE
Wavelength(s)	0.97885, 0.97885, 0.97896, 0.97118
Spacegroup name	P 21 21 21
Unit cell lengths	46.524, 70.129, 79.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.212
Rwork	0.210
R-free	0.26100
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.204
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.10)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.050	0.317
Number of reflections	38292
<I/σ(I)>	13.3
Completeness [%]	99.3	98.8
Redundancy	6.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	278	100 mM Na/MES/Acetate, pH 5.5, 24 % pentaerythritol ethoxylate (Mr 797), 300 mM NaCl, and 5% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 278K
1	VAPOR DIFFUSION, HANGING DROP	5.5	278	100 mM Na/MES/Acetate, pH 5.5, 24 % pentaerythritol ethoxylate (Mr 797), 300 mM NaCl, and 5% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 278K
1	VAPOR DIFFUSION, HANGING DROP	5.5	278	100 mM Na/MES/Acetate, pH 5.5, 24 % pentaerythritol ethoxylate (Mr 797), 300 mM NaCl, and 5% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 278K