2O02
Phosphorylation independent interactions between 14-3-3 and Exoenzyme S: from structure to pathogenesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97883 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.330, 72.238, 125.664 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.740 - 1.500 |
R-factor | 0.151 |
Rwork | 0.147 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.032 |
RMSD bond angle | 2.558 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.600 | |
High resolution limit [Å] | 1.500 | 10.000 | 1.500 |
Rmerge | 0.074 | 0.019 | 0.314 |
Number of reflections | 103943 | ||
<I/σ(I)> | 16.21 | 66.9 | 3.62 |
Completeness [%] | 99.5 | 92.5 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 285 | pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K |