2NZY

Crystal Structure of alpha1,3-Fucosyltransferase with GDP-fucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	103.962, 135.740, 96.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.050
R-factor	0.192
Rwork	0.190
R-free	0.24600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2nzw
RMSD bond length	0.020
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.080	0.656
Number of reflections	83859
<I/σ(I)>	22.5	2.1
Completeness [%]	97.9	92.2
Redundancy	6.1	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	4-5% PEG 3350, 0.2-0.4M ammonium phosphate, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K