2NZV
Structural mechanism for the fine-tuning of CcpA function by the small molecule effectors G6P and FBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-23 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.770, 69.770, 231.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.530 - 3.000 |
R-factor | 0.259 |
Rwork | 0.259 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nzu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.800 | 3.190 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.085 | 0.328 |
Number of reflections | 12099 | |
<I/σ(I)> | 6 | 1.8 |
Completeness [%] | 94.9 | 94 |
Redundancy | 3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | ammomium sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |