2NZO
Crystal structure of a secretion chaperone CsaA from Bacillus subtilis in the space group P 32 2 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 102 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 148.432, 148.432, 54.054 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.050 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gd7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.777 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.050 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.310 |
| Total number of observations | 18968 | |
| Number of reflections | 45675 | |
| <I/σ(I)> | 20.4 | 4.6 |
| Completeness [%] | 98.6 | 97 |
| Redundancy | 4.58 | 4.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M Potassium dihydrogen phosphate, 12% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
