2NZI
Crystal structure of domains A168-A170 from titin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 125.890, 125.890, 134.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.2191 |
| Rwork | 0.219 |
| R-free | 0.27350 |
| Structure solution method | MAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 26792 | |
| <I/σ(I)> | 22.7 | 3.6 |
| Completeness [%] | 96.6 | 98.6 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 2.1M NaCl, 0.1M MES, 0.6M Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
