2NZC
The structure of uncharacterized protein TM1266 from Thermotoga maritima.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-10 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97924, 0.97938 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.245, 81.448, 108.877 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.430 - 1.950 |
| R-factor | 0.21052 |
| Rwork | 0.208 |
| R-free | 0.25943 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.467 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.400 | 2.001 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 22928 | |
| <I/σ(I)> | 7 | |
| Completeness [%] | 99.1 | 98.51 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1 M ammonium dihydrogen phosphate, 0.1M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
