2NZ2
Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.95373 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 95.940, 117.470, 155.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.410 - 2.400 |
R-factor | 0.19757 |
Rwork | 0.194 |
R-free | 0.26475 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vl2 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.858 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.152 | 0.268 |
Number of reflections | 17358 | |
<I/σ(I)> | 3.98 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.3 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 16% PEG 3350, 0.15M DL-MALIC ACID, pH 7.0, VAPOR DIFFUSION, temperature 293K |