2NYZ

Viral Chemokine Binding Protein M3 From Murine Gammaherpesvirus68 In Complex With The C- Chemokine XCL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	173
Detector technology	CCD
Collection date	2001-06-22
Detector	ADSC QUANTUM 315
Spacegroup name	I 21 21 21
Unit cell lengths	84.370, 103.190, 290.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 2.600
R-factor	0.219
Rwork	0.219
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ml0
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.760
High resolution limit [Å]	2.600	2.600
Number of reflections	35559
<I/σ(I)>	16.1	2.7
Completeness [%]	90.0	79.2
Redundancy	7	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	10% PEG 8000, 100 mM TRIS, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K