2NYT

The APOBEC2 Crystal Structure and Functional Implications for AID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Collection date	2006-01-15
Wavelength(s)	0.9774, 0.9796, 0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	37.841, 89.410, 245.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.246
R-free	0.29500
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.018
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.500	2.500
Rmerge	0.151	0.333
Number of reflections	30088
<I/σ(I)>	17.7	2.5
Completeness [%]	96.3	85.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291.15	85mM NaCitrate, 160mM LiSO4, 24% (wt/vol) PEG, 15% glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
1	VAPOR DIFFUSION, HANGING DROP	5.6	291.15	85mM NaCitrate, 160mM LiSO4, 24% (wt/vol) PEG, 15% glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K