2NYF
Crystal structure of phenylalanine ammonia-lyase from Nostoc punctiforme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 74.279, 91.486, 151.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.500 |
Rwork | 0.247 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.340 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.098 | 0.475 |
Number of reflections | 16902 | |
<I/σ(I)> | 12.56 | 2.8 |
Completeness [%] | 92.9 | 69.7 |
Redundancy | 4.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M MOPSO (pH 7.0), 21% (w/v) polyethylene glycol 8000, 0.2 M lithium sulfate, 2 mM dithiothreitol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |