2NYE
Crystal structure of the Bateman2 domain of yeast Snf4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 235.618, 235.618, 235.618 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.450 - 2.500 |
| R-factor | 0.239 |
| Rwork | 0.238 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nyc |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 19934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 294 | 30% (w/v) PEG4000, 200 mM (NH4)Formate, and 3% Benzamidine, pH 4.5, VAPOR DIFFUSION, temperature 294K |
