2NYE

Crystal structure of the Bateman2 domain of yeast Snf4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-06
Detector	ADSC QUANTUM 4
Spacegroup name	F 4 3 2
Unit cell lengths	235.618, 235.618, 235.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.450 - 2.500
R-factor	0.239
Rwork	0.238
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nyc
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500	2.500
Number of reflections	19934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	294	30% (w/v) PEG4000, 200 mM (NH4)Formate, and 3% Benzamidine, pH 4.5, VAPOR DIFFUSION, temperature 294K