2NYB
Crystal structure of E.Coli Iron Superoxide Dismutase Q69E at 1.1 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.88557 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.508, 107.596, 84.120 |
| Unit cell angles | 90.00, 94.89, 90.00 |
Refinement procedure
| Resolution | 30.290 - 1.100 |
| R-factor | 0.16 |
| Rwork | 0.160 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.067 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.290 |
| High resolution limit [Å] | 1.100 |
| Number of reflections | 283939 |
| <I/σ(I)> | 28 |
| Completeness [%] | 100.0 |
| Redundancy | 1.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 100 mM Ammonium acetate pH 5.7, 10 mM Sodium citrate, 22.5% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
