2NY5
HIV-1 gp120 Envelope Glycoprotein (M95W, W96C, I109C, T257S, V275C, S334A, S375W, Q428C, A433M) Complexed with CD4 and Antibody 17b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 73.262, 87.643, 197.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.970 - 2.500 |
| R-factor | 0.2007 |
| Rwork | 0.198 |
| R-free | 0.25488 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gc1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.883 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.163 | 0.075 | 0.478 |
| Number of reflections | 37534 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 84.9 | 97.4 | 62.9 |
| Redundancy | 3.9 | 4.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 9.2% PEG 4000, 9.5% MPD, 100 mM Na Citrate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
