2NY3
HIV-1 gp120 Envelope Glycoprotein (K231C, T257S, E267C, S334A, S375W) Complexed with CD4 and Antibody 17b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-13 |
| Detector | MAR CCD 225 mm |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 71.740, 88.197, 197.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.110 - 2.000 |
| R-factor | 0.20513 |
| Rwork | 0.203 |
| R-free | 0.24284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gc1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.962 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.108 | 0.056 | 0.496 |
| Number of reflections | 81583 | ||
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 95.6 | 99.6 | 85.9 |
| Redundancy | 5.3 | 6.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 8.5% PEG 4000, 8.5% MPD, 100 mM Na Citrate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
