2NXP
Structure of NTD2 domain of the human TAF5 subunit of TFIID
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2005-10-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9797, 1.0199 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.620, 61.829, 133.357 |
| Unit cell angles | 90.00, 105.16, 90.00 |
Refinement procedure
| Resolution | 64.420 - 2.170 |
| R-factor | 0.21507 |
| Rwork | 0.213 |
| R-free | 0.26252 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.722 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.400 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.102 | 0.065 |
| Number of reflections | 76736 | |
| <I/σ(I)> | 9.3 | 2.5 |
| Completeness [%] | 97.8 | 97.9 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.1 | 295 | 0.1M Tris-HCl, 0.6M calcium chloride, 20% PEG 4000, pH 7.1, VAPOR DIFFUSION, temperature 295K |
