2NXE
T. thermophilus ribosomal protein L11 methyltransferase (PrmA) in complex with S-Adenosyl-L-Methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | H 3 |
Unit cell lengths | 187.179, 187.179, 45.189 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.750 |
R-factor | 0.20265 |
Rwork | 0.201 |
R-free | 0.22396 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nxc |
RMSD bond length | 0.015 |
RMSD bond angle | 1.705 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | COMO |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.046 | 0.434 |
Number of reflections | 59391 | |
<I/σ(I)> | 19.13 | 2.1 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 2.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 277 | 190 mM Calcium chloride dihydrate, 95mM HEPES-Na, pH 7.5, 26.6% v/v PEG400, 5% glycerol, microbatch under oil, temperature 277K |