2NX9
Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.825, 91.655, 116.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.700 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rqh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.123 | 0.578 |
| Number of reflections | 105661 | |
| <I/σ(I)> | 13.8 | 2.47 |
| Completeness [%] | 98.5 | 95.5 |
| Redundancy | 7.14 | 5.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 0.1 M Na-cacodylate 0.2 M (NH4)2SO4 5% glycerol 25% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.50 |
