2NX8

The crystal structure of the tRNA-specific adenosine deaminase from Streptococcus pyogenes

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	77
Detector technology	CCD
Collection date	2004-12-08
Detector	ADSC QUANTUM 210
Wavelength(s)	1.2834, 1.2827, 1.2573, 1.28
Spacegroup name	P 42 21 2
Unit cell lengths	80.091, 80.091, 81.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.660 - 2.000
R-factor	0.214
Rwork	0.214
R-free	0.24200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.081	0.081
Number of reflections	30630
<I/σ(I)>	29.6
Completeness [%]	89.9	89.9
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M HEPES, 2% PEG 1000, 1.7M Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K