2NX0
Ferrous nitrosyl blackfin tuna myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.520, 51.530, 38.060 |
| Unit cell angles | 90.00, 105.86, 90.00 |
Refinement procedure
| Resolution | 21.810 - 0.950 |
| R-factor | 0.15673 |
| Rwork | 0.156 |
| R-free | 0.17805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1myt |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.728 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.000 |
| High resolution limit [Å] | 0.950 |
| Number of reflections | 79652 |
| <I/σ(I)> | 12 |
| Completeness [%] | 98.7 |
| Redundancy | 9.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M potassium/sodium tartrate, 0.1 M sodium citrate, 2.0 M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
