2NWU
Crystal structure of protein SSO1042 from Sulfolobus solfataricus, Pfam DUF54
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-10 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.680, 66.307, 124.746 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.440 - 2.400 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.29800 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.058 | 0.231 |
| Number of reflections | 16881 | |
| <I/σ(I)> | 16.4 | 2.4 |
| Completeness [%] | 97.9 | 97.9 |
| Redundancy | 14.2 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | Sodium Citrate, Isoporpanol, PEG4000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
