2NWL
Crystal structure of GltPh in complex with L-Asp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Spacegroup name | P 61 |
| Unit cell lengths | 115.296, 115.296, 323.781 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.960 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xfh |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.849 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.500 |
| High resolution limit [Å] | 2.960 | 3.400 |
| Number of reflections | 15137 | |
| <I/σ(I)> | 17.1 | 2 |
| Completeness [%] | 89.9 | 47.8 |
| Redundancy | 5.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
