2NWH
Carbohydrate kinase from Agrobacterium tumefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-13 |
Detector | SBC-3 |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 75.930, 75.930, 103.608 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.700 - 1.860 |
R-factor | 0.1966 |
Rwork | 0.194 |
R-free | 0.23310 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.548 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.700 | 1.910 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.906 | |
Number of reflections | 29531 | |
<I/σ(I)> | 41.3 | 2.04 |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 16.2 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M calcium chloride, 7% PEG 3350, 4% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |