2NWD
Structure of chemically synthesized human lysozyme at 1 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.261, 61.133, 32.851 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.040 |
R-factor | 0.13361 |
Rwork | 0.133 |
R-free | 0.15222 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jsf see ref. K. Harada Y. abe M. Muraki Proteins 1998 30 232. |
RMSD bond length | 0.017 |
RMSD bond angle | 1.716 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.040 |
Number of reflections | 53675 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | c 6 mg/mL, 60 mM LiCl, 1.25 mM Hepes, 15 mM sodium phosphate, 1.25 M NaCl , pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |