2NWC
A 3.02 angstrom crystal structure of wild-type apo GroEL in a monoclinic space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 136.423, 262.254, 147.074 |
| Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.020 |
| R-factor | 0.22841 |
| Rwork | 0.227 |
| R-free | 0.26269 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kp8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.130 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Number of reflections | 202060 | |
| <I/σ(I)> | 9.8 | 1.02 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277 | 0.1 M imidazole, 35% MPD, 0.2 M MgCl2, pH 8.0, VAPOR DIFFUSION, temperature 277K |
