2NWA
X-ray Crystal Structure of Protein ytmB from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR466
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.662, 110.739, 60.672 |
| Unit cell angles | 90.00, 111.06, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.700 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| R-free | 0.28500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.328 |
| Number of reflections | 40226 | |
| <I/σ(I)> | 17.8 | 2.77 |
| Completeness [%] | 98.9 | 98.3 |
| Redundancy | 4.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Hepes, 0.2M NaI, 19% PEG3350, 5mM DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
