2NVP
X-Ray Crystal Structure of Protein CPF_0428 from Clostridium perfringens. Northeast Structural Genomics Consortium Target CpR63.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.857, 47.741, 84.376 |
| Unit cell angles | 90.00, 96.03, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.200 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.062 | 0.240 |
| Number of reflections | 53940 | |
| <I/σ(I)> | 0.056 | 6.2 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | 100mM MES, 20% PEG 8k, 100 mM magnesium acetate, 5mM DTT, pH 6.0, microbatch under oil, temperature 277K |
