2NVO

Crystal structure of Deinococcus radiodurans RO (RSR) protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Detector technology	CCD
Collection date	2005-08-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97934, 0.97926, 0.97939, 0.94927
Spacegroup name	C 1 2 1
Unit cell lengths	103.556, 87.610, 70.623
Unit cell angles	90.00, 96.57, 90.00

Refinement procedure

Resolution	46.600 - 1.890
R-factor	0.223
Rwork	0.223
R-free	0.26200
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.710
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.600	1.960
High resolution limit [Å]	1.890	1.890
Rmerge	0.062	0.685
Number of reflections	48610
<I/σ(I)>	18.9	1.64
Completeness [%]	98.0	85.3
Redundancy	3.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	0.1Mimidazole, 12% PEG 8000,0.2M calcium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K