2NVG
Soluble domain of Rieske Iron Sulfur protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 4 |
| Unit cell lengths | 69.935, 69.935, 54.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.350 |
| R-factor | 0.1429 |
| Rwork | 0.142 |
| R-free | 0.15929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nuk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.058 | 0.235 |
| Number of reflections | 29270 | |
| <I/σ(I)> | 29 | 3.8 |
| Completeness [%] | 100.0 | 55.8 |
| Redundancy | 5.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 276 | 1.5 M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 276K |
