2NVE
Soluble domain of Rieske Iron Sulfur Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | I 4 |
Unit cell lengths | 70.648, 70.648, 54.717 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.000 - 1.500 |
R-factor | 0.13635 |
Rwork | 0.133 |
R-free | 0.17370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nuk |
RMSD bond length | 0.008 |
RMSD bond angle | 1.406 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.056 | 0.199 |
Number of reflections | 20919 | |
<I/σ(I)> | 21 | 8 |
Completeness [%] | 96.7 | 87.7 |
Redundancy | 3.8 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 276 | 1.5 M ammonium sulfate, 20% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 276K |