2NV1
Structure of the synthase subunit Pdx1 (YaaD) of PLP synthase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 101.055, 106.202, 182.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.680 - 2.080 |
| R-factor | 0.14414 |
| Rwork | 0.142 |
| R-free | 0.19139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1znn |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.506 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 116594 | |
| <I/σ(I)> | 9 | 2.1 |
| Completeness [%] | 98.6 | 99.5 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 15-20% ethanol, 200mM MgCl2, 100mM Tris, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
