2NV1
Structure of the synthase subunit Pdx1 (YaaD) of PLP synthase from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 101.055, 106.202, 182.332 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.680 - 2.080 |
R-factor | 0.14414 |
Rwork | 0.142 |
R-free | 0.19139 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1znn |
RMSD bond length | 0.017 |
RMSD bond angle | 1.506 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.080 | 2.080 |
Number of reflections | 116594 | |
<I/σ(I)> | 9 | 2.1 |
Completeness [%] | 98.6 | 99.5 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 15-20% ethanol, 200mM MgCl2, 100mM Tris, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |