2NUU
Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97972 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.691, 107.865, 280.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.17506 |
| Rwork | 0.171 |
| R-free | 0.24942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1U7G |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.425 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 105009 | |
| <I/σ(I)> | 13.3 | 3 |
| Redundancy | 4.17 | 4.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 65% MPD, 100mM Tris pH8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
