2NUM
Soluble domain of Rieske Iron-Sulfur Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Spacegroup name | I 4 |
| Unit cell lengths | 70.626, 70.626, 54.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.500 |
| R-factor | 0.15357 |
| Rwork | 0.152 |
| R-free | 0.16726 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.334 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.610 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.051 | 0.156 |
| Number of reflections | 19979 | |
| <I/σ(I)> | 5.1 | 15 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 14.1 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 278 | 1.5 M ammonium sulfate, 20% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
