2NUK
Soluble Domain of the Rieske Iron-Sulfur Protein from Rhodobacter sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 70.568, 70.568, 54.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.670 - 1.200 |
| R-factor | 0.11969 |
| Rwork | 0.118 |
| R-free | 0.13639 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nwf |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.738 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.020 | 0.065 |
| Number of reflections | 41254 | |
| <I/σ(I)> | 24.7 | 19.6 |
| Completeness [%] | 98.0 | 99.7 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 278 | 1.5 M Ammonium sulfate, 20% anhydrous glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
