2NUI
X-ray Structure of synthetic [D83A]RNase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.590, 63.590, 63.365 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.100 |
| R-factor | 0.17773 |
| Rwork | 0.177 |
| R-free | 0.19319 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1izp |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.625 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.050 |
| Number of reflections | 59512 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.89 | 0.1 M Bis-Tris, 1.0 M ammonium sulfate, 1.5 M NaCl, pH 5.89, Drop 1:1 well:protein solution in H2O, VAPOR DIFFUSION, HANGING DROP |
