2NUH
Crystal structure of CutA from the phytopathgen bacterium Xylella fastidiosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.43 |
Spacegroup name | P 63 |
Unit cell lengths | 70.590, 70.590, 35.502 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.390 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vhf |
RMSD bond length | 0.011 |
RMSD bond angle | 1.465 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.080 | 1.440 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.085 | 0.424 |
Number of reflections | 19947 | |
<I/σ(I)> | 15.2 | 3.2 |
Completeness [%] | 97.2 | 82.7 |
Redundancy | 6.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M Mes Buffer, 1.6M Ammonium sulfate, 10% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |