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2NUC

STAPHLOCOCCAL NUCLEASE, ETHANE THIOL DISULFIDE TO V23C VARIANT

Experimental procedure
Source typeROTATING ANODE
Source detailsRIGAKU RUH2R
Temperature [K]278.15
Detector technologyIMAGE PLATE
Collection date1996-01-15
DetectorMACSCIENCE
Spacegroup nameP 41
Unit cell lengths48.500, 48.500, 63.200
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution6.000 - 2.000
R-factor0.179
Rwork0.179
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1snc
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR
Refinement softwareX-PLOR
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]6.0002.080
High resolution limit [Å]2.0002.000
Rmerge0.054
Number of reflections9282
Completeness [%]89.077
Redundancy5.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

8.154

*

Loll, P.J., (1989) Proteins Struct. Funct. Genet., 5, 183.

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein20 (mg/ml)
21droppotassium phosphate10.5 (mM)
31dropMPD17 (%(w/w))
41drop10 (mM)
51droppotassium citrate20 (mM)
61reservoirpotassium phosphate10.5 (mM)
71reservoirMPD21-23 (%(w/w))

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