2NTX
Prone8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 114.339, 114.339, 318.964 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.740 - 2.200 |
R-factor | 0.24496 |
Rwork | 0.244 |
R-free | 0.27030 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.580 |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.300 | |
High resolution limit [Å] | 2.200 | 15.000 | 2.200 |
Rmerge | 0.085 | 0.037 | 0.452 |
Number of reflections | 62938 | ||
<I/σ(I)> | 20.98 | 47.1 | 4.2 |
Completeness [%] | 99.2 | 92 | 95.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 740mM NaCl, 100mM sodium citrate, pH 5.6, vapor diffusion, hanging drop, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 740mM NaCl, 100mM sodium citrate, pH 5.6, vapor diffusion, hanging drop, temperature 293K |