2NTE
Crystal Structure of the BARD1 BRCT Domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9784 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.441, 75.797, 116.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.870 - 1.900 |
R-factor | 0.19871 |
Rwork | 0.196 |
R-free | 0.24743 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.865 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 40185 | |
<I/σ(I)> | 30.7 | 4.3 |
Completeness [%] | 98.3 | 91.7 |
Redundancy | 7.7 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM MES, 20% PEG 8K, 150mM Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |