2NTC
Crystal Structure of sv40 large T antigen origin binding domain with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.489, 68.341, 97.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.840 - 2.400 |
| R-factor | 0.2088 |
| Rwork | 0.205 |
| R-free | 0.28692 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | t antigen origin binding domain and four base pairs duplex dna |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.013 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.100 |
| Number of reflections | 15314 |
| <I/σ(I)> | 2.1 |
| Completeness [%] | 90.1 |
| Redundancy | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 273 | 0.1 M sodium cacodylate pH 6.5, 0.1 M calcium acetate, 30 % v/v peg 8000, 20 % glycerol, VAPOR DIFFUSION, temperature 273K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 1 | calcium acetate | ||
| 3 | 1 | 1 | peg 8000 | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 2 | sodium cacodylate | ||
| 6 | 1 | 2 | calcium acetate | ||
| 7 | 1 | 2 | peg 8000 | ||
| 8 | 1 | 2 | glycerol |
