2NT3
Receiver domain from Myxococcus xanthus social motility protein FrzS (Y102A Mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-15 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 63 |
| Unit cell lengths | 63.804, 63.804, 46.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| R-factor | 0.13352 |
| Rwork | 0.132 |
| R-free | 0.16244 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gkg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.590 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.093 | 0.837 |
| Number of reflections | 26709 | |
| <I/σ(I)> | 12 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 0.1M MIB buffer pH 7.0, 25% w/v PEG 1500, VAPOR DIFFUSION, temperature 291K |
