2NSO
Trna-gunanine-transglycosylase (TGT) mutant Y106F, C158V, A232S, V233G- APO-Structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Detector technology | IMAGE PLATE |
Collection date | 2004-09-13 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.730, 64.830, 70.810 |
Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
Resolution | 28.420 - 1.600 |
Rwork | 0.162 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y5x |
RMSD bond length | 0.009 |
RMSD bond angle | 0.027 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.133 |
Number of reflections | 53101 |
<I/σ(I)> | 15 |
Completeness [%] | 98.6 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 100 mM Tris/HCl, 1 mM DTT, 5% PEG 8000, 10% DMSO, pH 8.50 |