2NSO
Trna-gunanine-transglycosylase (TGT) mutant Y106F, C158V, A232S, V233G- APO-Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-13 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.730, 64.830, 70.810 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 28.420 - 1.600 |
| Rwork | 0.162 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y5x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.027 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.133 |
| Number of reflections | 53101 |
| <I/σ(I)> | 15 |
| Completeness [%] | 98.6 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 mM Tris/HCl, 1 mM DTT, 5% PEG 8000, 10% DMSO, pH 8.50 |
