2NSN
Crystal structure of Caspace Activation and Recruitment Domain (CARD) of NOD1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 2006-07-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 40.141, 40.141, 150.748 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.780 - 2.000 |
R-factor | 0.222 |
Rwork | 0.221 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dgn |
RMSD bond length | 0.012 |
RMSD bond angle | 1.340 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.6L) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.380 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.059 | 0.355 |
Total number of observations | 11350 | |
Number of reflections | 8946 | |
<I/σ(I)> | 21.3 | 4.8 |
Completeness [%] | 99.3 | 99.5 |
Redundancy | 13.26 | 13.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.7 | 277 | 15% PEG 3000, 100mM acetate, 20mgs/ml protein, pH 4.7, VAPOR DIFFUSION, temperature 277K |