2NSI
HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE, ZN-FREE, SEITU COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 188.370, 188.370, 230.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.23800 |
| Structure solution method | OTHER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.230 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.092 * | 0.699 * |
| Total number of observations | 355712 * | |
| Number of reflections | 82161 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 98.9 | 98.4 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5 | 7 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | GSH | 2.0 (mM) | |
| 3 | 1 | drop | L-Arg | 10 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 35 (%sat) | |
| 5 | 1 | reservoir | glycerol | 30 (%(v/v)) | |
| 6 | 1 | reservoir | sodium citrate | 50 (mM) |
