2NS9
Crystal structure of protein APE2225 from Aeropyrum pernix K1, Pfam COXG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-27 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97950 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.730, 33.082, 78.442 |
Unit cell angles | 90.00, 121.40, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.21974 |
Rwork | 0.217 |
R-free | 0.27752 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.916 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 1.930 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.077 | 0.327 |
Number of reflections | 32448 | |
<I/σ(I)> | 10.7 | 2.1 |
Completeness [%] | 99.0 | 100 |
Redundancy | 6.9 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 22% PEG 3350, 0.2M Ammonium Acetate, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |