2NS6
Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CAMD BEAMLINE GCPCC |
Synchrotron site | CAMD |
Beamline | GCPCC |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-22 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 47.967, 47.967, 165.927 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
Rwork | 0.238 |
R-free | 0.28700 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.205 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.046 | 0.170 |
Number of reflections | 11903 | |
<I/σ(I)> | 17.1 | 10.5 |
Completeness [%] | 97.8 | 92.9 |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10 | 277 | 21-27% PEG4000, 0.1 M CAPS, 0.2 M NaCl, 10 mM MnCl2, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 10 | 277 | 21-27% PEG4000, 0.1 M CAPS, 0.2 M NaCl, 10 mM MnCl2, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |